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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated TIP47 Monoclonal antibody

TIP47 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4C11B1

Cat no : CL555-66523

Synonyms

47 kDa MPR binding protein, Cargo selection protein TIP47, M6PRBP1, Perilipin 3, Placental protein 17, PLIN3, PP17, TIP47

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL555-66523

    FC experiment of HeLa using CL555-66523

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human TIP47 (CL555-66523, Clone:4C11B1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66523-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL555-66523 targets TIP47 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIP47 fusion protein Ag1028 相同性解析による交差性が予測される生物種
Full Name mannose-6-phosphate receptor binding protein 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007566
Gene symbol M6PRBP1
Gene ID (NCBI) 10226
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Protocols

Product Specific Protocols
FC protocol for CL555 TIP47 antibody CL555-66523Download protocol
Standard Protocols
Click here to view our Standard Protocols