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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TIMELESS Monoclonal antibody

TIMELESS Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6G3

Cat no : CL488-67022

Synonyms

FLJ12640, FLJ20714, hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1

Validation Data Gallery

Applications:
  • FC experiment of HEK-293 using CL488-67022

    FC experiment of HEK-293 using CL488-67022

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TIMELESS (CL488-67022, Clone:1B6G3) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67022-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67022 targets TIMELESS in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIMELESS fusion protein Ag6283 相同性解析による交差性が予測される生物種
Full Name timeless homolog (Drosophila)
Calculated molecular weight 139 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC050557
Gene symbol TIMELESS
Gene ID (NCBI) 8914
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TIMELESS antibody CL488-67022Download protocol
Standard Protocols
Click here to view our Standard Protocols