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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIMELESS Monoclonal antibody

TIMELESS Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6G3

Cat no : 67022-1-Ig

Synonyms

FLJ12640, FLJ20714, hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1

Validation Data Gallery

Applications:
  • WB analysis using 67022-1-Ig

    WB analysis using 67022-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • WB analysis of NIH/3T3 using 67022-1-Ig

    WB analysis of NIH/3T3 using 67022-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67022-1-Ig

    WB analysis of A549 using 67022-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of JAR using 67022-1-Ig

    WB analysis of JAR using 67022-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67022-1-Ig

    WB analysis of Jurkat using 67022-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67022-1-Ig

    WB analysis of HSC-T6 using 67022-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 67022-1-Ig

    WB analysis of U2OS using 67022-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 67022-1-Ig

    FC experiment of HEK-293 using 67022-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, A549 cells, JAR cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells
Positive FC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67022-1-Ig targets TIMELESS in WB, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIMELESS fusion protein Ag6283 相同性解析による交差性が予測される生物種
Full Name timeless homolog (Drosophila)
Calculated molecular weight 139 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC050557
Gene symbol TIMELESS
Gene ID (NCBI) 8914
RRIDAB_2882337
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Protocols

Product Specific Protocols
WB protocol for TIMELESS antibody 67022-1-IgDownload protocol
FC protocol for TIMELESS antibody 67022-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,canineWB

Eur J Pharmacol

TIMELESS promotes the proliferation and migration of lung adenocarcinoma cells by activating EGFR through AMPK and SPHK1 regulation

Authors - Houqing Yin
  • KD Validated
View Article