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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

THOC6 Polyclonal antibody

THOC6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15316-1-AP

Synonyms

fSAP35, THO complex subunit 6 homolog, THOC6, WDR58

Validation Data Gallery

Applications:
  • WB analysis using 15316-1-AP

    WB analysis using 15316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse ovary using 15316-1-AP

    WB analysis of mouse ovary using 15316-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 15316-1-AP

    IP experiment of A431 using 15316-1-AP

    IP result of anti-THOC6 (IP:15316-1-AP, 4ug; Detection:15316-1-AP 1:300) with A431 cells lysate 2280 ug.

  • IHC staining of human ovary tumor using 15316-1-AP

    IHC staining of human ovary tumor using 15316-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15316-1-AP (THOC6 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, mouse ovary tissue, Neuro-2a cells
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15316-1-AP targets THOC6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOC6 fusion protein Ag7561 相同性解析による交差性が予測される生物種
Full Name THO complex 6 homolog (Drosophila)
Calculated molecular weight 38 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC003118
Gene symbol THOC6
Gene ID (NCBI) 79228
RRIDAB_2240482
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for THOC6 antibody 15316-1-APDownload protocol
IHC protocol for THOC6 antibody 15316-1-APDownload protocol
IP protocol for THOC6 antibody 15316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Viruses

Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.

Authors - Toshie Sakuma
  • KD Validated
View Article