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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TFAP2A,AP-2 Monoclonal antibody

TFAP2A,AP-2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B12F11

Cat no : CL488-67076

Synonyms

Activator protein 2, AP 2, AP 2 transcription factor, AP 2alpha, AP2, AP2 alpha, AP2TF, BOFS, FLJ51761, TFAP2, TFAP2A

Validation Data Gallery

Applications:
  • FC experiment of A431 using CL488-67076

    FC experiment of A431 using CL488-67076

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TFAP2A,AP-2 (CL488-67076, Clone:1B12F11) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67076-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67076 targets TFAP2A,AP-2 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TFAP2A,AP-2 fusion protein Ag4112 相同性解析による交差性が予測される生物種
Full Name transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC017754
Gene symbol TFAP2A
Gene ID (NCBI) 7020
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression. [PMID:21966377] TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation [PMID:11694877]

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TFAP2A,AP-2 antibody CL488-67076Download protocol
Standard Protocols
Click here to view our Standard Protocols