TARS Monoclonal antibody
TARS Monoclonal Antibody for WB, IF, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1A11F10
Cat no : 67828-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, PC-12 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
67828-1-Ig targets TARS in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TARS fusion protein Ag6614 相同性解析による交差性が予測される生物種 |
| Full Name | threonyl-tRNA synthetase |
| Calculated molecular weight | 83 kDa |
| Observed molecular weight | 83-87 kDa |
| GenBank accession number | BC000517 |
| Gene symbol | TARS |
| Gene ID (NCBI) | 6897 |
| RRID | AB_2918590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TARS antibody 67828-1-Ig | Download protocol |
| IF protocol for TARS antibody 67828-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. |
