TAP1 Monoclonal antibody
TAP1 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B8A7
Cat no : 68412-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A2780 cells, SW 1990 cells, HeLa cels, MDA-MB-231 cells, HaCaT cells, THP-1 cells, Daudi cells, Ramos cells, Raji cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Product Information
68412-1-Ig targets TAP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TAP1 fusion protein Ag21303 相同性解析による交差性が予測される生物種 |
Full Name | transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) |
Calculated molecular weight | 81 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC014081 |
Gene symbol | TAP1 |
Gene ID (NCBI) | 6890 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for TAP1 antibody 68412-1-Ig | Download protocol |
IHC protocol for TAP1 antibody 68412-1-Ig | Download protocol |
IF protocol for TAP1 antibody 68412-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |