• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Syntenin-1 Monoclonal antibody

Syntenin-1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B1A9

Cat no : CL488-68096

Synonyms

MDA 9, MDA9, Scaffold protein Pbp1, SDCBP, ST1, SYCL, Syndecan binding protein 1, Syntenin 1, TACIP18

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL488-68096

    FC experiment of HepG2 using CL488-68096

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Syntenin-1 (CL488-68096, Clone:2B1A9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SDCBP Ready-To-Use IHC Kit. SDCBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68096-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-68096 targets Syntenin-1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntenin-1 fusion protein Ag18078 相同性解析による交差性が予測される生物種
Full Name syndecan binding protein (syntenin)
Calculated molecular weight 298 aa, 32 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC113674
Gene symbol SDCBP
Gene ID (NCBI) 6386
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Syntenin-1 antibody CL488-68096Download protocol
Standard Protocols
Click here to view our Standard Protocols