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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SUMO2/3 Polyclonal antibody

SUMO2/3 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11251

Synonyms

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, Ubiquitin like protein SMT3B

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using CL488-11251

    IF Staining of HEK-293 using CL488-11251

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 SUMO2/3 antibody (CL488-11251) at dilution of 1:200.

Tested Applications

Positive IF detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 11251-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-11251 targets SUMO2/3 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUMO2/3 fusion protein Ag1778 相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Calculated molecular weight 11 kDa
Observed molecular weight 11-20 kDa
GenBank accession numberBC016775
Gene symbol SUMO2
Gene ID (NCBI) 6613
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SUMO2/3 antibody CL488-11251Download protocol
Standard Protocols
Click here to view our Standard Protocols