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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin-conjugated STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for WB, IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Biotin

Cat no : Biotin-11459

Synonyms

EIEE4, FLJ37475, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

Validation Data Gallery

Applications:
  • WB analysis of Y79 using Biotin-11459

    WB analysis of Y79 using Biotin-11459

    Y79 cells were subjected to SDS PAGE followed by western blot with Biotin-11459 (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using Biotin-11459

    IHC staining of mouse brain using Biotin-11459

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-11459 (STXBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

Biotin-11459 targets STXBP1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STXBP1 fusion protein Ag1978 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 1
Calculated molecular weight 594 aa, 68 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC015749
Gene symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_2918937
Conjugate Biotin
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
IHC protocol for Biotin STXBP1 antibody Biotin-11459Download protocol
Standard Protocols
Click here to view our Standard Protocols