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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STUB1 Monoclonal antibody

STUB1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B9

Cat no : 68407-1-Ig

Synonyms

Antigen NY CO 7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1

Validation Data Gallery

Applications:
  • WB analysis using 68407-1-Ig

    WB analysis using 68407-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68407-1-Ig (STUB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of MCF-7 using 68407-1-Ig

    IF Staining of MCF-7 using 68407-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using STUB1 antibody (68407-1-Ig, Clone: 2E6B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy STUB1 Ready-To-Use IHC Kit. STUB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68407-1-Ig targets STUB1 in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name STIP1 homology and U-box containing protein 1
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberNM_005861
Gene symbol STUB1
Gene ID (NCBI) 10273
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.

Protocols

Product Specific Protocols
WB protocol for STUB1 antibody 68407-1-IgDownload protocol
IF protocol for STUB1 antibody 68407-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols