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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SSSCA1 Monoclonal antibody

SSSCA1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D2E8

Cat no : 68423-1-Ig

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

Validation Data Gallery

Applications:
  • WB analysis using 68423-1-Ig

    WB analysis using 68423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68423-1-Ig

    IF Staining of HeLa using 68423-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68423-1-Ig targets SSSCA1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SSSCA1 fusion protein Ag16783 相同性解析による交差性が予測される生物種
Full Name Sjogren syndrome/scleroderma autoantigen 1
Calculated molecular weight 199 aa, 21 kDa
Observed molecular weight23 kDa
GenBank accession numberBC014791
Gene symbol SSSCA1
Gene ID (NCBI) 10534
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

Protocols

Product Specific Protocols
WB protocol for SSSCA1 antibody 68423-1-IgDownload protocol
IF protocol for SSSCA1 antibody 68423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols