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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SRF Monoclonal antibody

SRF Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G8F4

Cat no : CL594-66742

Synonyms

MCM1, Serum response factor, SRF

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66742

    IF Staining of HepG2 using CL594-66742

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66742 (SRF antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66742-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66742 targets SRF in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SRF fusion protein Ag10386 相同性解析による交差性が予測される生物種
Full Name serum response factor (c-fos serum response element-binding transcription factor)
Calculated molecular weight 508 aa, 52 kDa
GenBank accession numberBC048211
Gene symbol SRF
Gene ID (NCBI) 6722
RRIDAB_2883622
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Protocols

Product Specific Protocols
IF protocol for CL594 SRF antibody CL594-66742Download protocol
Standard Protocols
Click here to view our Standard Protocols