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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

SRF Monoclonal antibody

SRF Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G8F4

Cat no : 66742-1-Ig

Synonyms

MCM1, Serum response factor, SRF

Validation Data Gallery

Applications:
  • WB analysis using 66742-1-Ig

    WB analysis using 66742-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66742-1-Ig

    WB analysis of MCF-7 using 66742-1-Ig

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.

  • WB analysis of HeLa using 66742-1-Ig

    WB analysis of HeLa using 66742-1-Ig

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells.

  • WB analysis of RAW 264.7 using 66742-1-Ig

    WB analysis of RAW 264.7 using 66742-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66742-1-Ig

    IF Staining of HepG2 using 66742-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 66742-1-Ig

    IF Staining of HepG2 using 66742-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, MCF-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66742-1-Ig targets SRF in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SRF fusion protein Ag10386 相同性解析による交差性が予測される生物種
Full Name serum response factor (c-fos serum response element-binding transcription factor)
Calculated molecular weight 508 aa, 52 kDa
GenBank accession numberBC048211
Gene symbol SRF
Gene ID (NCBI) 6722
RRIDAB_2882090
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Protocols

Product Specific Protocols
WB protocol for SRF antibody 66742-1-IgDownload protocol
IF protocol for SRF antibody 66742-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis

Authors - Ni Zeng
View Article