SQRDL Monoclonal antibody
SQRDL Monoclonal Antibody for WB, IP, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, pig
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1B4E3
Cat no : 68459-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, HT-29 cells, hTERT-RPE1 cells, THP-1 cells, pig liver tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Sample-dependent, check data in validation data gallery | |
Product Information
68459-1-Ig targets SQRDL in WB, IP, ELISA applications and shows reactivity with Human, pig samples.
| Tested Reactivity | Human, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SQRDL fusion protein Ag10762 相同性解析による交差性が予測される生物種 |
| Full Name | sulfide quinone reductase-like (yeast) |
| Calculated molecular weight | 450 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC016836 |
| Gene symbol | SQRDL |
| Gene ID (NCBI) | 58472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SQRDL antibody 68459-1-Ig | Download protocol |
| IP protocol for SQRDL antibody 68459-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
