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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2C8

Cat no : CL488-66786

Synonyms

DOM, SOX10, Transcription factor SOX 10, WS2E, WS4

Validation Data Gallery

Applications:
  • IF Staining of mouse brain using CL488-66786

    IF Staining of mouse brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • IF Staining of rat brain using CL488-66786

    IF Staining of rat brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • FC experiment of C6 using CL488-66786

    FC experiment of C6 using CL488-66786

    1X10^6 C6 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SOX10 (CL488-66786, Clone:1D2C8) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inmouse brain tissue, rat brain tissue
Positive FC detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66786-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66786 targets SOX10 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOX10 fusion protein Ag23191 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 10
Calculated molecular weight 50 kDa
GenBank accession numberBC002824
Gene symbol SOX10
Gene ID (NCBI) 6663
RRIDAB_2919369
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SOX10 antibody CL488-66786Download protocol
FC protocol for CL Plus 488 SOX10 antibody CL488-66786Download protocol
Standard Protocols
Click here to view our Standard Protocols