Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, MDA-MB-453 cells |
| Positive IHC-Autostainer detected in | human appendicitis tissue, human skin cancer tissue, human malignant melanoma tissue, human breast tissue |
| Positive IHC detected in | human malignant melanoma tissue, human pituitary adenoma tissue, mouse brain tissue, rat cerebellum tissue, human breast tissue, human skin cancer tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive FC (Intra) detected in | C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 8 publications below |
| IF | See 18 publications below |
Product Information
66786-1-Ig targets SOX10 in WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, chicken, zebrafish |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23191 Product name: Recombinant human SOX10 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 267-466 aa of BC002824 Sequence: GKPHIDFGNVDIGEISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 10 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC002824 |
| Gene Symbol | SOX10 |
| Gene ID (NCBI) | 6663 |
| RRID | AB_2882131 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56693 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842).SOX10 sumoylation by UBC9 (sumo-conjugating enzyme UBC9) is the best described one. Occurring at lysines K55, K246 and K357,19 it inhibits NC development and promotes development of non-sensory cranial placodes in vivo.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| IF protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| IHC protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| WB protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Brown adipose tissue secretes OLFM4 to coordinate sensory and sympathetic innervation via Schwann cells | ||
Nucleic Acids Res SRSF10 regulates oligodendrocyte differentiation during mouse central nervous system development by modulating pre-mRNA splicing | ||
J Neuroinflammation SIRT6 modulates lesion microenvironment in LPC induced demyelination by targeting astrocytic CHI3L1 |