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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMS Monoclonal antibody

SMS Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8E9

Cat no : 68040-1-Ig

Synonyms

MRSR, SMS, spermine synthase, SPMSY, SpS, SRS

Validation Data Gallery

Applications:
  • WB analysis using 68040-1-Ig

    WB analysis using 68040-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68040-1-Ig (SMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68040-1-Ig

    IF Staining of HeLa using 68040-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SMS antibody (68040-1-Ig, Clone: 1G8E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 68040-1-Ig

    FC experiment of HeLa using 68040-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMS (68040-1-Ig, Clone:1G8E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, HCT 116 cells, HEK-293 cells, Hela cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

68040-1-Ig targets SMS in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMS fusion protein Ag8840 相同性解析による交差性が予測される生物種
Full Name spermine synthase
Calculated molecular weight 366 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC009898
Gene symbol SMS
Gene ID (NCBI) 6611
RRIDAB_2918782
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SMS antibody 68040-1-IgDownload protocol
IF protocol for SMS antibody 68040-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols