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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMO Monoclonal antibody

SMO Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Canine

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A11A10

Cat no : 66851-2-Ig

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

Validation Data Gallery

Applications:
  • IF Staining of MDCK using 66851-2-Ig

    IF Staining of MDCK using 66851-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using SMO antibody (66851-2-Ig, Clone: 2A11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

Tested Applications

Positive IF detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy SMO Ready-To-Use IHC Kit. SMO primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66851-2-Ig targets SMO in IF, ELISA applications and shows reactivity with Human, Canine samples.

Tested Reactivity Human, Canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMO fusion protein Ag25861 相同性解析による交差性が予測される生物種
Full Name smoothened homolog (Drosophila)
Calculated molecular weight 787 aa, 86 kDa
GenBank accession numberBC009989
Gene symbol SMO
Gene ID (NCBI) 6608
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Protocols

Product Specific Protocols
IF protocol for SMO antibody 66851-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols