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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC5A8 Polyclonal antibody

SLC5A8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29827-1-AP

Synonyms

AIT, Apical iodide transporter, SLC5A8, SMCT, SMCT1

Validation Data Gallery

Applications:
  • WB analysis using 29827-1-AP

    WB analysis using 29827-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29827-1-AP (SLC5A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 29827-1-AP

    IHC staining of rat brain using 29827-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 29827-1-AP

    IHC staining of rat brain using 29827-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29827-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29827-1-AP targets SLC5A8 in WB, IHC, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A8 fusion protein Ag30183 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (iodide transporter), member 8
Calculated molecular weight 610 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC110492
Gene symbol SLC5A8
Gene ID (NCBI) 160728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 69-kDa protein functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in the healthy human, rat, and mouse colon. And the protein is localized to apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.

Protocols

Product Specific Protocols
WB protocol for SLC5A8 antibody 29827-1-APDownload protocol
IHC protocol for SLC5A8 antibody 29827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols