• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NKCC1,SLC12A2 Polyclonal antibody

NKCC1,SLC12A2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28055-1-AP

Synonyms

Basolateral Na K Cl symporter, BSC, BSC2, NKCC1, SLC12A2

Validation Data Gallery

Applications:
  • WB analysis using 28055-1-AP

    WB analysis using 28055-1-AP

    Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28055-1-AP (SLC12A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28055-1-AP

    IHC staining of human breast cancer using 28055-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (SLC12A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 28055-1-AP

    IHC staining of human breast cancer using 28055-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (SLC12A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 28055-1-AP

    IHC staining of human prostate cancer using 28055-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28055-1-AP (SLC12A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 28055-1-AP

    IHC staining of human prostate cancer using 28055-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28055-1-AP (SLC12A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 28055-1-AP

    IF Staining of HeLa using 28055-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NKCC1,SLC12A2 antibody (28055-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inunboiled HeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28055-1-AP targets NKCC1,SLC12A2 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKCC1,SLC12A2 fusion protein Ag27895 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (sodium/potassium/chloride transporters), member 2
Calculated molecular weight 131 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC033003
Gene symbol SLC12A2
Gene ID (NCBI) 6558
RRIDAB_2881048
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

Protocols

Product Specific Protocols
WB protocol for NKCC1,SLC12A2 antibody 28055-1-APDownload protocol
IHC protocol for NKCC1,SLC12A2 antibody 28055-1-APDownload protocol
IF protocol for NKCC1,SLC12A2 antibody 28055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols