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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SKIV2L Monoclonal antibody

SKIV2L Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9G2

Cat no : 68547-1-Ig

Synonyms

170A, DDX13, Helicase like protein, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, SKIV2L, W

Validation Data Gallery

Applications:
  • WB analysis using 68547-1-Ig

    WB analysis using 68547-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, NK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

68547-1-Ig targets SKIV2L in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SKIV2L fusion protein Ag33679 相同性解析による交差性が予測される生物種
Full Name superkiller viralicidic activity 2-like (S. cerevisiae)
Calculated molecular weight 1246 aa, 138 kDa
Observed molecular weight138-145 kDa
GenBank accession numberBC015758
Gene symbol SKIV2L
Gene ID (NCBI) 6499
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

Protocols

Product Specific Protocols
WB protocol for SKIV2L antibody 68547-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols