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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHH Polyclonal antibody

SHH Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 29752-1-AP

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

Validation Data Gallery

Applications:
  • WB analysis using 29752-1-AP

    WB analysis using 29752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 29752-1-AP

    WB analysis using 29752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 29752-1-AP

    IHC staining of mouse embryo using 29752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 29752-1-AP

    IHC staining of mouse embryo using 29752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 29752-1-AP

    FC experiment of HeLa using 29752-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHH (29752-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

29752-1-AP targets SHH in WB, IHC, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHH fusion protein Ag31338 相同性解析による交差性が予測される生物種
Full Name sonic hedgehog homolog (Drosophila)
Calculated molecular weight 50 kDa
Observed molecular weight50-60 kDa
GenBank accession numberNM_000193
Gene symbol SHH
Gene ID (NCBI) 6469
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHH, Sonic hedgehog protein, binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PMID: 10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PMID: 10753901). SHH is synthesized as a 45 kDa precursor that undergoes an autocatalytic processing event that produces a 19 kDa N-terminal product, responsible for all signaling activities, and a 25 kDa C-terminal fragment (PMID: 10753901, PMID: 16282375).

Protocols

Product Specific Protocols
WB protocol for SHH antibody 29752-1-APDownload protocol
IHC protocol for SHH antibody 29752-1-APDownload protocol
FC protocol for SHH antibody 29752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols