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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SH3BP1 Monoclonal antibody

SH3BP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B12C6

Cat no : 68328-1-Ig

Synonyms

3BP 1, SH3 domain binding protein 1, SH3BP1

Validation Data Gallery

Applications:
  • WB analysis using 68328-1-Ig

    WB analysis using 68328-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68328-1-Ig

    WB analysis of LNCaP using 68328-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68328-1-Ig

    WB analysis of HepG2 using 68328-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHSC-T6 cells, LNCaP cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68328-1-Ig targets SH3BP1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SH3BP1 fusion protein Ag15165 相同性解析による交差性が予測される生物種
Full Name SH3-domain binding protein 1
Calculated molecular weight 701 aa, 76 kDa
Observed molecular weight76-80 kDa
GenBank accession numberBC008282
Gene symbol SH3BP1
Gene ID (NCBI) 23616
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3BP1 is also named as Chimeric SH3BP1-PDXP protein and BARGIN, and belongs to the to the RhoGAP family. SH3BP1 can be as a partner of the exocyst complex and establish a physical and functional link between two motility-driving pathways, the Ral/exocyst and Rac signaling pathways. (PMID:21658605). SH3BP1 localizes together with the exocyst to the leading edge of motile cells and that SH3BP1 regulates cell migration via its GAP activity upon Rac1.

Protocols

Product Specific Protocols
WB protocol for SH3BP1 antibody 68328-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols