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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SF4 Monoclonal antibody

SF4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G9D8

Cat no : 68319-1-Ig

Synonyms

DKFZp434E2216, F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4

Validation Data Gallery

Applications:
  • WB analysis using 68319-1-Ig

    WB analysis using 68319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of NCCIT using 68319-1-Ig

    WB analysis of NCCIT using 68319-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU2OS cells, NCCIT cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68319-1-Ig targets SF4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SF4 fusion protein Ag8014 相同性解析による交差性が予測される生物種
Full Name splicing factor 4
Calculated molecular weight 72 kDa
Observed molecular weight72 kDa
GenBank accession numberBC002986
Gene symbol SF4
Gene ID (NCBI) 57794
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SF4 antibody 68319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols