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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SENP2 Polyclonal antibody

SENP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29772-1-AP

Synonyms

AXAM2, DKFZp762A2316, KIAA1331, SENP2, Sentrin specific protease 2, SMT3 specific isopeptidase 2, SMT3IP2

Validation Data Gallery

Applications:
  • WB analysis using 29772-1-AP

    WB analysis using 29772-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29772-1-AP (SENP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 29772-1-AP

    IHC staining of human pancreas cancer using 29772-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29772-1-AP (SENP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inEC109 cells, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

29772-1-AP targets SENP2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SENP2 fusion protein Ag30728 相同性解析による交差性が予測される生物種
Full Name SUMO1/sentrin/SMT3 specific peptidase 2
Calculated molecular weight 68 kDa
Observed molecular weight60-68 kDa
GenBank accession numberNM_021627
Gene symbol SENP2
Gene ID (NCBI) 59343
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SENP2 (Sentrin-specific protease 2), as a protease, catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PMID:15296745). The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PMID:20194620, PMID:21965678). It has 2 isoforms with the molecular weight of 66 and 68 kDa.

Protocols

Product Specific Protocols
WB protocol for SENP2 antibody 29772-1-APDownload protocol
IHC protocol for SENP2 antibody 29772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols