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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCLY Monoclonal antibody

SCLY Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3A2G7

Cat no : 67606-1-Ig

Synonyms

hSCL, SCL, SCLY, selenocysteine lyase

Validation Data Gallery

Applications:
  • WB analysis using 67606-1-Ig

    WB analysis using 67606-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67606-1-Ig (SCLY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67606-1-Ig

    IHC staining of human breast cancer using 67606-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67606-1-Ig (SCLY antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67606-1-Ig

    IHC staining of human breast cancer using 67606-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67606-1-Ig (SCLY antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, L02 cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67606-1-Ig targets SCLY in WB, IHC,ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SCLY fusion protein Ag28841 相同性解析による交差性が予測される生物種
Full Name selenocysteine lyase
Calculated molecular weight 49 kDa
Observed molecular weight48 kDa
GenBank accession numberBC007891
Gene symbol SCLY
Gene ID (NCBI) 51540
RRIDAB_2882812
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SCLY antibody 67606-1-IgDownload protocol
IHC protocol for SCLY antibody 67606-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols