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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL6 Monoclonal antibody

RPL6 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3F6F8

Cat no : 67729-1-Ig

Synonyms

60S ribosomal protein L6, Neoplasm related protein C140, ribosomal protein L6, RPL6, SHUJUN 2, TAXREB107, TXREB1

Validation Data Gallery

Applications:
  • WB analysis using 67729-1-Ig

    WB analysis using 67729-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67729-1-Ig (RPL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 67729-1-Ig

    IF Staining of L02 using 67729-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using RPL6 antibody (67729-1-Ig, Clone: 3F6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inL02 cells
Planning an IHC experiment? We recommend our IHCeasy RPL6 Ready-To-Use IHC Kit. RPL6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67729-1-Ig targets RPL6 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPL6 fusion protein Ag6840 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L6
Calculated molecular weight 33 kDa
Observed molecular weight34 kDa
GenBank accession numberBC004138
Gene symbol RPL6
Gene ID (NCBI) 6128
RRIDAB_2882915
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].

Protocols

Product Specific Protocols
WB protocol for RPL6 antibody 67729-1-IgDownload protocol
IF protocol for RPL6 antibody 67729-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols