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  • Featured Product
  • KD/KO Validated

Kazrin Polyclonal antibody

Kazrin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11572-1-AP

Synonyms

KAZ, kazrin, KIAA1026, RP1 21O18.1, RP1-21O18.1

Validation Data Gallery

Applications:
  • WB analysis of human heart using 11572-1-AP

    WB analysis of human heart using 11572-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis using 11572-1-AP

    WB analysis using 11572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11572-1-AP

    WB analysis using 11572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (Kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 11572-1-AP

    WB analysis of mouse brain using 11572-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 11572-1-AP

    WB analysis of mouse heart using 11572-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 11572-1-AP

    WB analysis of rat heart using 11572-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11572-1-AP

    IHC staining of human colon cancer using 11572-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11572-1-AP (kazrin antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with Kazrin antibody included
  • IHC staining of mouse skin using 11572-1-AP

    IHC staining of mouse skin using 11572-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Kazrin antibody included
  • IHC staining of mouse skin using 11572-1-AP

    IHC staining of mouse skin using 11572-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Kazrin antibody included

Tested Applications

Positive WB detected inmouse skin tissue, mouse heart tissue, human heart tissue, mouse brain tissue, rat heart tissue, rat skin tissue
Positive IHC detected inhuman colon cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Kazrin Ready-To-Use IHC Kit. Kazrin primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11572-1-AP targets Kazrin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kazrin fusion protein Ag2152 相同性解析による交差性が予測される生物種
Full Name kazrin
Calculated molecular weight 415 aa, 46 kDa
Observed molecular weight 46 kDa, 37 kDa
GenBank accession numberBC035501
Gene symbol RP1-21O18.1
Gene ID (NCBI) 23254
RRIDAB_833126
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kazrin is an evolutionarily conserved protein that is upregulated during keratinocyte terminal differentiation. Kazrin localizes to desmosomes and binds the epidermal cornified envelope protein periplakin. Kazrin exists three isoforms:kazrin A (46.7 kDa), kazrin B (46 kDa), and kazrin C (37 kDa).

Protocols

Product Specific Protocols
WB protocol for Kazrin antibody 11572-1-APDownload protocol
IHC protocol for Kazrin antibody 11572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

J Invest Dermatol

Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.

Authors - Chhatriwala Mariya K MK
  • KD Validated
View Article