CoraLite®594-conjugated ROR1 Monoclonal antibody
ROR1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1F5D8
Cat no : CL594-66923
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL594-66923 targets ROR1 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ROR1 fusion protein Ag14586 相同性解析による交差性が予測される生物種 |
| Full Name | receptor tyrosine kinase-like orphan receptor 1 |
| Calculated molecular weight | 937 aa, 104 kDa |
| GenBank accession number | BC006374 |
| Gene symbol | ROR1 |
| Gene ID (NCBI) | 4919 |
| RRID | AB_2920057 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ROR1 is a member of the RTK family of orphan receptors related to muscle specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937-amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 ROR1 antibody CL594-66923 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
