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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIP3 Polyclonal antibody

RIP3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29080-1-AP

Synonyms

Receptor interacting protein 3, RIP 3, RIP like protein kinase 3, RIP3, RIPK3

Validation Data Gallery

Applications:
  • WB analysis using 29080-1-AP

    WB analysis using 29080-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 29080-1-AP (RIP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy RIPK3 Ready-To-Use IHC Kit. RIPK3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

29080-1-AP targets RIP3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIP3 fusion protein Ag30337 相同性解析による交差性が予測される生物種
Full Name receptor-interacting serine-threonine kinase 3
Calculated molecular weight 518 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC062584
Gene symbol RIPK3
Gene ID (NCBI) 11035
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIPK3,also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is recruited to the TNFR1 signaling complex through RIP and has been shown to mediate apoptosis induction and NF-κB activation. RIPK3 is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus(PMID:18533105). It has 3 isoforms produced by alternative splicing. RIPK3 might form a homodimer within cells, and its apoptotic activity may not be required for this dimerization(PMID:18533105).

Protocols

Product Specific Protocols
WB protocol for RIP3 antibody 29080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols