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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RHOA Monoclonal antibody

RHOA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B3D7

Cat no : CL488-66733

Synonyms

ARH12, ARHA, h12, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-66733

    IF Staining of HeLa using CL488-66733

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66733 (RHOA antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RHOA Ready-To-Use IHC Kit. RHOA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66733-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66733 targets RHOA in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHOA fusion protein Ag1141 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member A
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005976
Gene symbol RHOA
Gene ID (NCBI) 387
RRIDAB_2883376
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RHOA antibody CL488-66733Download protocol
Standard Protocols
Click here to view our Standard Protocols