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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

REL Monoclonal antibody

REL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A4

Cat no : 67747-1-Ig

Synonyms

C Rel, Proto oncogene c Rel, REL

Validation Data Gallery

Applications:
  • WB analysis using 67747-1-Ig

    WB analysis using 67747-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 67747-1-Ig

    WB analysis of HEK-293 using 67747-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inDaudi cells, HEK-293 cells, Jurkat cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

67747-1-Ig targets REL in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen REL fusion protein Ag26803 相同性解析による交差性が予測される生物種
Full Name v-rel reticuloendotheliosis viral oncogene homolog (avian)
Calculated molecular weight 69 kDa
Observed molecular weight68 kDa, 80 kDa
GenBank accession numberNM_001291746
Gene symbol REL
Gene ID (NCBI) 5966
RRIDAB_2918516
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REL, also known as, Proto-oncogene c-Rel, is a 619 amino acid protein, which may play a role in differentiation and lymphopoiesis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. The full-length 80-kDa c-Rel was identified (PMID: 9857058).

Protocols

Product Specific Protocols
WB protocol for REL antibody 67747-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols