REG4 Polyclonal antibody

REG4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12268-1-AP

Synonyms

GISP, REG 4, REG IV, REG like protein, REG4, RELP



Tested Applications

Positive WB detected inSGC-7901 cells, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12268-1-AP targets REG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REG4 fusion protein Ag2934 相同性解析による交差性が予測される生物種
Full Name regenerating islet-derived family, member 4
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC017089
Gene symbol REG4
Gene ID (NCBI) 83998
RRIDAB_2178702
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for REG4 antibody 12268-1-APDownload protocol
IHC protocol for REG4 antibody 12268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Genomics Proteomics

Regulation of REG4 Expression and Prediction of 5-Fluorouracil Sensitivity by CDX2 in Ovarian Mucinous Carcinoma.

Authors - Iemasa Koh
humanIHC

J Surg Oncol

Overexpression of REG4 confers an independent negative prognosticator in rectal cancers receiving concurrent chemoradiotherapy.

Authors - Hong-Lin He
humanIHC

Signal Transduct Target Ther

Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis.

Authors - Xiaobo Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other