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Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using REG4 Polyclonal antibody (12268-1-AP)

    WB analysis of SGC-7901 using 12268-1-AP (same clone as 12268-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12268-1-AP (REG4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12268-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using REG4 Polyclonal antibody (12268-1-AP)

    WB analysis of mouse liver using 12268-1-AP (same clone as 12268-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12268-1-AP (REG4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12268-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using REG4 Polyclonal antibody (12268-1-AP)

    IHC staining of human colon cancer using 12268-1-AP (same clone as 12268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12268-1-AP (REG4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12268-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using REG4 Polyclonal antibody (12268-1-AP)

    IHC staining of human colon cancer using 12268-1-AP (same clone as 12268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12268-1-AP (REG4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12268-1-PBS in a different storage buffer formulation.

REG4 Polyclonal antibody, PBS Only

REG4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12268-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GISP, REG 4, Reg IV, REG like protein, REG-4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12268-1-PBS targets REG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2934

Product name: Recombinant human REG4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-158 aa of BC017089

Sequence: VLGDIIMRPSCAPGWFYHKSNCYGYFRKLRNWSDAELECQSYGNGAHLASILSLKEASTIAEYISGYQRSQPIWIGLHDPQKRQQWQWIDGAMYLYRSWSGKSMGGNKHCAEMSSNNNFLTWSSNECNKRQHFLCKYRP

相同性解析による交差性が予測される生物種
Full Name regenerating islet-derived family, member 4
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC017089
Gene Symbol REG4
Gene ID (NCBI) 83998
RRIDAB_2178702
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYZ8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

REG4 (Regenerating islet-derived protein 4) is a secreted C-type lectin that belongs to the regenerating gene (REG) family. Initially identified in a cDNA library from ulcerative colitis tissue, REG4 is predominantly expressed by intestinal enteroendocrine cells and is markedly up-regulated during gut inflammation and in various gastrointestinal cancers.

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