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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RBM4 Monoclonal antibody

RBM4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H3F12

Cat no : CL488-60292

Synonyms

DKFZp547K0918, hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-60292

    IF Staining of HeLa using CL488-60292

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RBM4 antibody (CL488-60292, Clone: 3H3F12 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60292-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60292 targets RBM4 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBM4 fusion protein Ag2190 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 4
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC021120
Gene symbol RBM4
Gene ID (NCBI) 5936
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RBM4 antibody CL488-60292Download protocol
Standard Protocols
Click here to view our Standard Protocols