RBAP48 Polyclonal antibody
RBAP48 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 14557-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, mouse thymus tissue |
| Positive IHC detected in | human ovary tissue, human brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RBBP4 Ready-To-Use IHC Kit. RBBP4 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
14557-1-AP targets RBAP48 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RBAP48 fusion protein Ag6110 相同性解析による交差性が予測される生物種 |
| Full Name | retinoblastoma binding protein 4 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 40 kDa, 48-53 kDa |
| GenBank accession number | BC053904 |
| Gene symbol | RBBP4 |
| Gene ID (NCBI) | 5928 |
| RRID | AB_2177630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBAP48 antibody 14557-1-AP | Download protocol |
| IHC protocol for RBAP48 antibody 14557-1-AP | Download protocol |
| IF protocol for RBAP48 antibody 14557-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
