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Target

Host

Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RASEF Polyclonal antibody

RASEF Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11569-1-AP

Synonyms

RAB45, Ras related protein Rab 45, RASEF

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 11569-1-AP

    WB analysis of mouse testis using 11569-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 11569-1-AP

    WB analysis of mouse heart using 11569-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11569-1-AP

    IHC staining of human pancreas cancer using 11569-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11569-1-AP (RASEF antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RASEF antibody included

Tested Applications

Positive WB detected inmouse testis tissue, mouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASEF Ready-To-Use IHC Kit. RASEF primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11569-1-AP targets RASEF in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASEF fusion protein Ag2149 相同性解析による交差性が予測される生物種
Full Name RAS and EF-hand domain containing
Calculated molecular weight 740 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC023566
Gene symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2238112
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RASEF antibody 11569-1-APDownload protocol
IHC protocol for RASEF antibody 11569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Mol Cancer Res

RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.

Authors - Oshita Hideto H
  • KD Validated
View Article