RALB Monoclonal antibody
RALB Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, rat, pig, mouse and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D11C11
Cat no : 67094-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HeLa cells, rat brain tissue, mouse brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, Pig brain cells |
| Positive IHC detected in | mouse bladder tissue, human lung cancer tissue, mouse liver tissue, mouse lung tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human lung cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy RALB Ready-To-Use IHC Kit. RALB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
67094-1-Ig targets RALB in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.
| Tested Reactivity | Human, rat, pig, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RALB fusion protein Ag28600 相同性解析による交差性が予測される生物種 |
| Full Name | v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) |
| Calculated molecular weight | 206 aa, 23 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC018163 |
| Gene symbol | RALB |
| Gene ID (NCBI) | 5899 |
| RRID | AB_2882399 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RALB antibody 67094-1-Ig | Download protocol |
| IHC protocol for RALB antibody 67094-1-Ig | Download protocol |
| IF protocol for RALB antibody 67094-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Pathol Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors | ||
Cancers (Basel) Controlled Plasma Membrane Delivery of FGFR1 and Modulation of Signaling by a Novel Regulated Anterograde RTK Transport Pathway |
