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RACGAP1 Monoclonal antibody

RACGAP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

6E10A2

Cat no : 66056-1-Ig

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 66056-1-Ig

    WB analysis of HEK-293 using 66056-1-Ig

    HEK-293, Jurkat, ROS1728, PC-12, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK293 cells using 66056-1-Ig

    WB analysis of HEK293 cells using 66056-1-Ig

    WB result of RACGAP1 antibody (66056-1-Ig, 1:3000) with si-control and si-RACGAP1 transfected HEK293 cells.

  • WB analysis of human brain using 66056-1-Ig

    WB analysis of human brain using 66056-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66056-1-Ig

    WB analysis of HeLa using 66056-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66056-1-Ig

    WB analysis of HEK-293 using 66056-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66056-1-Ig

    IHC staining of human colon cancer using 66056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66056-1-Ig

    IHC staining of human colon cancer using 66056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66056-1-Ig

    IF Staining of HeLa using 66056-1-Ig

    Immunofluorescent analysis of HeLa cells, using RACGAP1 antibody 66056-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, HEK293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66056-1-Ig is RACGAP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag6944 相同性解析による交差性が予測される生物種
Full Name Rac GTPase activating protein 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC032754
Gene symbol RACGAP1
Gene ID (NCBI) 29127
RRIDAB_11043178
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Protocols

Product Specific Protocols
WB protocol for RACGAP1 antibody 66056-1-IgDownload protocol
IHC protocol for RACGAP1 antibody 66056-1-IgDownload protocol
IF protocol for RACGAP1 antibody 66056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols