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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-TBC1D1 (Ser660) Recombinant antibody

Phospho-TBC1D1 (Ser660) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

7N19

Cat no : 81363-2-RR

Synonyms

KIAA1108, TBC, TBC1, TBC1 domain family member 1, TBC1D1

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 81363-2-RR

    WB analysis of NIH/3T3 using 81363-2-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

  • WB analysis of HeLa using 81363-2-RR

    WB analysis of HeLa using 81363-2-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

Tested Applications

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

81363-2-RR targets Phospho-TBC1D1 (Ser660) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Calculated molecular weight 133 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC050321
Gene symbol TBC1D1
Gene ID (NCBI) 23216
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)

Protocols

Product Specific Protocols
WB protocol for Phospho-TBC1D1 (Ser660) antibody 81363-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols