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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal antibody

Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C11D9

Cat no : 68041-1-Ig

Synonyms

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

Validation Data Gallery

Applications:
  • WB analysis using 68041-1-Ig

    WB analysis using 68041-1-Ig

    Non-treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control.

  • WB analysis using 68041-1-Ig

    WB analysis using 68041-1-Ig

    Non-treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control.

  • IF Staining of HeLa using 68041-1-Ig

    IF Staining of HeLa using 68041-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (68041-1-Ig, Clone: 2C11D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, λ phosphatase treated HeLa cells, λ phosphatase treated NIH/3T3 cells
Positive IF detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit. Phospho-RPS6KA1 (Ser380) primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68041-1-Ig targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Calculated molecular weight 735 aa, 83 kDa
Observed molecular weight80 kDa
GenBank accession numberBC014966
Gene symbol RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2918783
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)

Protocols

Product Specific Protocols
WB protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 68041-1-IgDownload protocol
IF protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 68041-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols