CoraLite® Plus 647-conjugated Phospho-RPS6KA1 (Ser380) Recombinant antibody
Phospho-RPS6KA1 (Ser380) Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
7F23
Cat no : CL647-80108
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | TPA treated Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.13 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL647-80108 targets Phospho-RPS6KA1 (Ser380) in FC (Intra) applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
| Calculated molecular weight | 735 aa, 83 kDa |
| Observed molecular weight | 83-90 kDa |
| GenBank accession number | BC014966 |
| Gene symbol | RPS6KA1 |
| Gene ID (NCBI) | 6195 |
| RRID | AB_2920329 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)
