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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Phospho-Caspase 9 (Ser196) Recombinant antibody

Phospho-Caspase 9 (Ser196) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3P16

Cat no : CL594-80346

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, CASPASE 9c, Caspase9, ICE LAP6, ICE like apoptotic protease 6, MCH6

Validation Data Gallery

Applications:
  • FC experiment of Hela using CL594-80346

    FC experiment of Hela using CL594-80346

    1X10^6 HeLa cells untreated (dashed lines) or Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Human Phospho-Caspase 9 (Ser196) (CL594-80346, Clone:3P16) (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive FC detected inCalyculin A treated Hela cells
For other applications, we recommend the unconjugated version of this antibody, 80346-1-RR

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-80346 targets Phospho-Caspase 9 (Ser196) in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name caspase 9, apoptosis-related cysteine peptidase
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa, 35 kDa
GenBank accession numberBC002452
Gene symbol CASP9
Gene ID (NCBI) 842
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46kDa precursor protein which can be cleaved into a 35kDa subunit and a 11kDa subunit. The phosphorylated type can be detected at 55kDa and 35kDa. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It can be phosphorylated by PKB/AKT1 at Ser196, this modification will downregulate its activity and decrease apoptosis. Akt phosphorylation site found in human caspase 9 is absent in mouse caspase 9.It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507)