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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Peripherin Monoclonal antibody

Peripherin Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10B3

Cat no : 66317-1-Ig

Synonyms

NEF4, Neurofilament 4, Peripherin, PRPH, PRPH1

Validation Data Gallery

Applications:
  • WB analysis of rat brain using 66317-1-Ig

    WB analysis of rat brain using 66317-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of human colon using 66317-1-Ig

    WB analysis of human colon using 66317-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat colon using 66317-1-Ig

    WB analysis of rat colon using 66317-1-Ig

    rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66317-1-Ig

    IHC staining of human colon using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens).

  • IHC staining of human brain using 66317-1-Ig

    IHC staining of human brain using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 66317-1-Ig

    IHC staining of human brain using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human colon using 66317-1-Ig

    IHC staining of human colon using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens).

  • FC experiment of PC-12 using 66317-1-Ig

    FC experiment of PC-12 using 66317-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of SH-SY5Y using 66317-1-Ig

    FC experiment of SH-SY5Y using 66317-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inrat brain tissue, human colon tissue, rat colon tissue
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-12 cells, SH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66317-1-Ig targets Peripherin in WB, IHC, FC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peripherin fusion protein Ag11116 相同性解析による交差性が予測される生物種
Full Name peripherin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight 50-54 kDa
GenBank accession numberBC032703
Gene symbol PRPH
Gene ID (NCBI) 5630
RRIDAB_2881698
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Peripherin antibody 66317-1-IgDownload protocol
IHC protocol for Peripherin antibody 66317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols