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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTRF Polyclonal antibody

PTRF Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30086-1-AP

Synonyms

Cavin 1, FKSG13, PTRF

Validation Data Gallery

Applications:
  • WB analysis using 30086-1-AP

    WB analysis using 30086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30086-1-AP (PTRF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 30086-1-AP

    IF Staining of HepG2 using 30086-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PTRF antibody (30086-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HT-1080 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTRF Ready-To-Use IHC Kit. PTRF primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

30086-1-AP targets PTRF in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTRF fusion protein Ag32786 相同性解析による交差性が予測される生物種
Full Name polymerase I and transcript release factor
Calculated molecular weight 43 kDa
Observed molecular weight55 kDa
GenBank accession numberBC008849
Gene symbol PTRF
Gene ID (NCBI) 284119
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transcription of RNA Pol I, which involves pausing of transcription by TTF1, and dissociation of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55 kDa. (PMID: 11139612 )

Protocols

Product Specific Protocols
WB protocol for PTRF antibody 30086-1-APDownload protocol
IF protocol for PTRF antibody 30086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols