PTPRO Monoclonal antibody

PTPRO Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F2B4

Cat no : 67000-1-Ig

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O



Tested Applications

Positive WB detected inpig kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67000-1-Ig targets PTPRO in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PTPRO fusion protein Ag8284 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, O
Calculated molecular weight 138 kDa
Observed molecular weight 160 kDa, 180-220 kDa
GenBank accession numberBC035960
Gene symbol PTPRO
Gene ID (NCBI) 5800
RRIDAB_2882317
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

Protocols

Product Specific Protocols
WB protocol for PTPRO antibody 67000-1-IgDownload protocol
IHC protocol for PTPRO antibody 67000-1-IgDownload protocol
IF protocol for PTPRO antibody 67000-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mediators Inflamm

MiR-6803-5p Promotes Cancer Cell Proliferation and Invasion via PTPRO/NF-κB Axis in Colorectal Cancer.

Authors - Shushan Yan