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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMB8 Monoclonal antibody

PSMB8 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A5B6

Cat no : 66759-1-Ig

Synonyms

beta5i, D6S216, D6S216E, LMP7, Low molecular mass protein 7, Macropain subunit C13, Proteasome component C13, Proteasome subunit beta 5i, Proteasome subunit beta type 8, PSMB5i, PSMB8, RING10, Y2

Validation Data Gallery

Applications:
  • WB analysis using 66759-1-Ig

    WB analysis using 66759-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 66759-1-Ig

    WB analysis of HSC-T6 using 66759-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66759-1-Ig

    IHC staining of human lung cancer using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of human lung cancer using 66759-1-Ig

    IHC staining of human lung cancer using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of mouse colon using 66759-1-Ig

    IHC staining of mouse colon using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of mouse stomach using 66759-1-Ig

    IHC staining of mouse stomach using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of rat colon using 66759-1-Ig

    IHC staining of rat colon using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of human oesophagus cancer using 66759-1-Ig

    IHC staining of human oesophagus cancer using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of rat stomach using 66759-1-Ig

    IHC staining of rat stomach using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of human liver cancer using 66759-1-Ig

    IHC staining of human liver cancer using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of human liver cancer using 66759-1-Ig

    IHC staining of human liver cancer using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IF Staining of human lung cancer using 66759-1-Ig

    IF Staining of human lung cancer using 66759-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66759-1-Ig

    IF Staining of human liver cancer using 66759-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of C2C12 using 66759-1-Ig

    IF Staining of C2C12 using 66759-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HeLa using 66759-1-Ig

    IF Staining of HeLa using 66759-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, Raji cells, Ramos cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human oesophagus cancer tissue, mouse colon tissue, mouse stomach tissue, rat colon tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, human liver cancer tissue, human lung cancer tissue, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB8 Ready-To-Use IHC Kit. PSMB8 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66759-1-Ig targets PSMB8 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB8 fusion protein Ag6780 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Calculated molecular weight 30 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC001114
Gene symbol PSMB8
Gene ID (NCBI) 5696
RRIDAB_2882105
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).

Protocols

Product Specific Protocols
WB protocol for PSMB8 antibody 66759-1-IgDownload protocol
IHC protocol for PSMB8 antibody 66759-1-IgDownload protocol
IF protocol for PSMB8 antibody 66759-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols