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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA4 Monoclonal antibody

PSMA4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rabbit, Rat, Mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E6E4

Cat no : 68203-1-Ig

Synonyms

HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4

Validation Data Gallery

Applications:
  • WB analysis using 68203-1-Ig

    WB analysis using 68203-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68203-1-Ig (PSMA4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 68203-1-Ig

    IHC staining of human pancreas cancer using 68203-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68203-1-Ig (PSMA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA4 antibody included
  • IF Staining of HeLa using 68203-1-Ig

    IF Staining of HeLa using 68203-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMA4 antibody (68203-1-Ig, Clone: 2E6E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, K-562 cells, Pig heart, Rabbit heart, Rat heart, Mouse heart
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMA4 Ready-To-Use IHC Kit. PSMA4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68203-1-Ig targets PSMA4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse samples.

Tested Reactivity Human, Pig, Rabbit, Rat, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PSMA4 fusion protein Ag32626 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 4
Calculated molecular weight 237 aa, 27 kDa
Observed molecular weight29 kDa
GenBank accession numberBC056249
Gene symbol PSMA4
Gene ID (NCBI) 5685
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMA4 antibody 68203-1-IgDownload protocol
IHC protocol for PSMA4 antibody 68203-1-IgDownload protocol
IF protocol for PSMA4 antibody 68203-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols