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  • Featured Product
  • KD/KO Validated

PRDX3 Recombinant antibody

PRDX3 Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1L11

Cat no : 81833-1-RR

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

Validation Data Gallery

Applications:
  • WB analysis using 81833-1-RR

    WB analysis using 81833-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81833-1-RR (PRX3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 81833-1-RR

    WB analysis of HEK-293 using 81833-1-RR

    WB result of PRDX3 antibody (81833-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

  • IHC staining of human lung cancer using 81833-1-RR

    IHC staining of human lung cancer using 81833-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDX3 antibody included
  • IHC staining of human lung cancer using 81833-1-RR

    IHC staining of human lung cancer using 81833-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDX3 antibody included
  • IF Staining of HepG2 using 81833-1-RR

    IF Staining of HepG2 using 81833-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 81833-1-RR

    IF Staining of HepG2 using 81833-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, A549 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

81833-1-RR targets PRDX3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRDX3 fusion protein Ag1062 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 3
Calculated molecular weight 27 kDa
Observed molecular weight28 kDa
GenBank accession numberBC007062
Gene symbol PRDX3
Gene ID (NCBI) 10935
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Protocols

Product Specific Protocols
WB protocol for PRDX3 antibody 81833-1-RRDownload protocol
IHC protocol for PRDX3 antibody 81833-1-RRDownload protocol
IF protocol for PRDX3 antibody 81833-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols